Supplementary material from "Wnt5b integrates Fak1a to mediate gastrulation cell movements via Rac1 and Cdc42"

Published on 2020-03-16T05:07:41Z (GMT) by
Focal adhesion kinase (FAK) mediates vital cellular pathways during development. Despite its necessity, how FAK regulates and integrates with other signals during early embryogenesis remains poorly understood. We found that the loss of Fak1a impaired epiboly, convergent extension and hypoblast cell migration in zebrafish embryos. We also observed a clear disturbance in cortical actin at the blastoderm margin and distribution of yolk syncytial nuclei. In addition, we investigated a possible link between Fak1a and a well-known gastrulation regulator, Wnt5b, and revealed that the overexpression of <i>fak1a</i> or <i>wnt5b</i> could cross-rescue convergence defects induced by a <i>wnt5b</i> or <i>fak1a</i> antisense morpholino (MO), respectively. Wnt5b and Fak1a were shown to converge in regulating Rac1 and Cdc42, which could synergistically rescue <i>wnt5b</i> and <i>fak1a</i> morphant phenotypes. Furthermore, we generated several alleles of <i>fak1a</i> mutants using CRISPR/Cas9, but those mutants only revealed mild gastrulation defects. However, injection of a subthreshold level of the <i>wnt5b</i> MO induced severe gastrulation defects in <i>fak1a</i> mutants, which suggested that the upregulated expression of <i>wnt5b</i> might complement the loss of Fak1a. Collectively, we demonstrated that a functional interaction between Wnt and FAK signalling mediates gastrulation cell movements via the possible regulation of Rac1 and Cdc42 and subsequent actin dynamics.

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Hung, I-Chen; Chen, Tsung-Ming; Lin, Jing-Ping; Tai, Yu-Ling; Shen, Tang-Long; Lee, Shyh-Jye (2020): Supplementary material from "Wnt5b integrates Fak1a to mediate gastrulation cell movements via Rac1 and Cdc42". The Royal Society. Collection. https://doi.org/10.6084/m9.figshare.c.4858827.v2