Supplementary material from "Natural diversity of CRISPR spacers of Thermus: evidence of local spacer acquisition and global spacer exchange"
Version 2 2019-03-14, 04:24
Version 1 2019-02-14, 09:57
Posted on 2019-03-14 - 04:24
We investigated the diversity of CRISPR spacers of Thermus communities from two locations in Italy, two in Chile and one location in Russia. Among the five sampling sites, a total of more than 7200 unique spacers belonging to different CRISPR–Cas systems types and subtypes were identified. Most of these spacers are not found in CRISPR arrays of sequenced Thermus strains. Comparison of spacer sets revealed that samples within the same area (separated by few to hundreds of metres) have similar spacer sets, which appear to be largely stable at least over the course of several years. While at further distances (hundreds of kilometres and more), the similarity of spacer sets is decreased, there are still multiple common spacers in Thermus communities from different continents. The common spacers can be reconstructed in identical or similar CRISPR arrays, excluding their independent appearance and suggesting an extensive migration of thermophilic bacteria over long distances. Several new Thermus phages were isolated in the sampling sites. Mapping of spacers to bacteriophage sequences revealed examples of local acquisition of spacers from some phages and distinct patterns of targeting of phage genomes by different CRISPR–Cas systems.This article is part of the theme issue ‘The ecology and evolution of prokaryotic CRISPR–Cas adaptive immune systems’.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Lopatina, Anna; Medvedeva, Sofia; Artamonova, Daria; Kolesnik, Matvey; Sitnik, Vasily; Ispolatov, Yaroslav; et al. (2019). Supplementary material from "Natural diversity of CRISPR spacers of Thermus: evidence of local spacer acquisition and global spacer exchange". The Royal Society. Collection. https://doi.org/10.6084/m9.figshare.c.4400771.v2
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (7)
AL
Anna Lopatina
SM
Sofia Medvedeva
DA
Daria Artamonova
MK
Matvey Kolesnik
VS
Vasily Sitnik
YI
Yaroslav Ispolatov
KS
Konstantin Severinov