Supplementary material from "KAP1 targets actively transcribed genomic loci to exert pleomorphic effects on RNA polymerase II activity"
Version 2 2020-01-23, 14:31Version 2 2020-01-23, 14:31
Version 1 2019-12-24, 09:01Version 1 2019-12-24, 09:01
Posted on 2020-01-23 - 14:31
KAP1 (KRAB-associated protein 1) is best known as a co-repressor responsible for inducing heterochromatin formation notably at transposable elements. However, it has also been observed to bind the transcription start site of actively expressed genes. To address this paradox, we characterized the protein interactome of KAP1 in the human K562 erythro-leukaemia cell line. We found that the regulator can associate with a wide range of nucleic acid binding proteins, nucleosome remodellers, chromatin modifiers and other transcription modulators. We further determined that KAP1 is recruited at actively transcribed polymerase II promoters, where its depletion resulted in pleomorphic effects, whether expression of these genes was normally constitutive or inducible, consistent with the breadth of possible KAP1 interactors.This article is part of the discussion meeting issue ‘Crossroads between transposons and gene regulation’.
CITE THIS COLLECTION
DataCite
DataCiteDataCite
3 Biotech3 Biotech
3D Printing in Medicine3D Printing in Medicine
3D Research3D Research
3D-Printed Materials and Systems3D-Printed Materials and Systems
4OR4OR
AAPG BulletinAAPG Bulletin
AAPS OpenAAPS Open
AAPS PharmSciTechAAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität HamburgAbhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)ABI Technik (German)
Academic MedicineAcademic Medicine
Academic PediatricsAcademic Pediatrics
Academic PsychiatryAcademic Psychiatry
Academic QuestionsAcademic Questions
Academy of Management DiscoveriesAcademy of Management Discoveries
Academy of Management JournalAcademy of Management Journal
Academy of Management Learning and EducationAcademy of Management Learning and Education
Academy of Management PerspectivesAcademy of Management Perspectives
Academy of Management ProceedingsAcademy of Management Proceedings
Academy of Management ReviewAcademy of Management Review
Kauzlaric, Annamaria; Jang, Suk Min; Morchikh, Mehdi; Cassano, Marco; Planet, Evarist; Benkirane, Monsef; et al. (2019). Supplementary material from "KAP1 targets actively transcribed genomic loci to exert pleomorphic effects on RNA polymerase II activity". The Royal Society. Collection. https://doi.org/10.6084/m9.figshare.c.4795818.v2