Supplementary material from "Development and testing of a novel killer–rescue self-limiting gene drive system in Drosophila melanogaster"
Posted on 2020-04-01 - 03:53
Here we report the development and testing of a novel self-limiting gene drive system, Killer–Rescue (K–R), in Drosophila melanogaster. This system is composed of an autoregulated Gal4 Killer (K) and a Gal4-activated Gal80 Rescue (R). Overexpression of Gal4 is lethal, but in the presence of R activation of Gal80 leads to much lower levels of Gal4 and R of lethality. We demonstrate that with a single 2 : 1 engineered to wild-type release, K drives R through the population and after nine generations, more than 98% of the population carry R and less than 2% of the population are wild-type flies. We discuss how this simple K–R gene drive system may be readily adapted for population replacement in a human health pest, Aedes aegypti, or for population suppression in an agricultural pest, Drosophila suzukii.
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Webster, Sophia H.; Vella, Michael R.; Scott, Maxwell J. (2020). Supplementary material from "Development and testing of a novel killer–rescue self-limiting gene drive system in Drosophila melanogaster". The Royal Society. Collection. https://doi.org/10.6084/m9.figshare.c.4915581.v1