Supplementary material from "Cell migration through three-dimensional confining pores: speed accelerations by deformation and recoil of the nucleus"
Version 2 2019-06-10, 09:51
Version 1 2019-05-27, 08:11
Posted on 2019-06-10 - 09:51
Directional cell migration in dense three-dimensional (3D) environments critically depends upon shape adaptation and is impeded depending on the size and rigidity of the nucleus. Accordingly, the nucleus is primarily understood as a physical obstacle; however, its pro-migratory functions by stepwise deformation and reshaping remain unclear. Using atomic force spectroscopy, time-lapse fluorescence microscopy and shape change analysis tools, we determined the nuclear size, deformability, morphology and shape change of HT1080 fibrosarcoma cells expressing the Fucci cell cycle indicator or being pre-treated with chromatin-decondensating agent TSA. We show oscillating peak accelerations during migration through 3D collagen matrices and microdevices that occur during shape reversion of deformed nuclei (recoil), and increase with confinement. During G1 cell-cycle phase, nucleus stiffness was increased and yielded further increased speed fluctuations together with sustained cell migration rates in confinement when compared to interphase populations, or to periods of intrinsic nuclear softening in the S/G2 cell-cycle phase. Likewise, nuclear softening by pharmacological chromatin decondensation or after lamin A/C depletion reduced peak oscillations in confinement. In conclusion, deformation and recoil of the stiff nucleus contributes to saltatory locomotion in dense tissues.This article is part of the discussion meeting issue ‘Forces in cancer: interdisciplinary approaches in tumour mechanobiology’.
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Krause, Marina; Yang, Feng Wei; Lindert, Mariska te; Isermann, Philipp; Schepens, Jan; Maas, Ralph J. A.; et al. (2019). Supplementary material from "Cell migration through three-dimensional confining pores: speed accelerations by deformation and recoil of the nucleus". The Royal Society. Collection. https://doi.org/10.6084/m9.figshare.c.4518938.v2
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AUTHORS (12)
MK
Marina Krause
FY
Feng Wei Yang
ML
Mariska te Lindert
PI
Philipp Isermann
JS
Jan Schepens
RM
Ralph J. A. Maas
CV
Chandrasekhar Venkataraman
JL
Jan Lammerding
AM
Anotida Madzvamuse
WH
Wiljan Hendriks
JR
Joost te Riet
KW
Katarina Wolf