Supplementary material from "A novel workflow for three-dimensional analysis of tumour cell migration"

The limitations of two-dimensional (2D) analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal-amoeboid transition led to the development of a workflow incorporating the generation and analysis of 3D data with Instant Structured Illumination Microscopy and a new ImageJ plugin.