Fig S4. Identification of labeled and infected labeled T lymphocytes using flow-cytometry from A large panel of chicken cells are invaded in vivo by Salmonella Typhimurium even when depleted of all known invasion factors

The anti-CD3 antibody, that allows T lymphocytes identification, was conjugated with FITC. Flow cytometric analyses were performed with a BD LSR Fortessa^TM X-20 (BD Biosciences, San Jose, CA, USA). BD FACSDiva^TM software (v 8.0.2) was used to analyze the cytometric data. For each sample, dot plots were analyzed. Debris were eliminated on the basis of morphological criteria, regions were set according to uninfected control samples and isotype-control staining. The intensity of green fluorescence (FITC) is on the vertical axis, plotted against the intensity of red fluorescence (TurboFP650) on the horizontal axis. Labeled cells emitting a green fluorescence were detected in the upper part of the graph. Infected labeled cells emitting both types of fluorescence (green and red) were revealed by dots in the upper right-hand part of the graph. Unlabeled infected cells could also be seen in the lower right-hand part of the graph. Results are expressed as percentages. Some examples are presented. Staining in the spleen is shown for the T lymphocytes.