Supplementary Video 3 from Geometric constraint of mechanosensing by modification of hydrogel thickness prevents stiffness-induced differentiation in bone marrow stromal cells

24-hour time-lapse videos, started ~24 hours after cell seeding of BMSCs cultivated at either low (1000/cm²) or high (10,000/cm²) cell density on soft, thick PAAm hydrogels. Phase contrast videos of cells are shown in the top panels and fluorescence videos on bead displacement are shown in the bottom panels. Displacements are greater in cells cultivated at low density, suggesting that cell-cell mechanical integration abrogates local cell-matrix deformation, possibly as tension is integrated across the entirety of the PAAm hydrogel.