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rsif20230779_si_001.mp4 (51.61 MB)

Animation of the field cricket posterior tympanum and tracheal branches with <10 µm cuticle colour-coded red indicating coupled membranes from Coupled membranes: A mechanism of frequency filtering and transmission in the field cricket ear evidenced by micro-computed tomography, laser Doppler vibrometry, and finite element analysis

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posted on 2024-04-02, 04:33 authored by Brendan Latham, Andrew Reid, Joseph Curt Jackson, Jonathan A. Williams, James F C Windmill
Animation S1. Animation of the field cricket tracheal branches and posterior tympanum revealing three coupled membranes in red. µ-CT volumetric visualisation followed by a segmented 3D thickness colour model showing cuticle below 10 µm in thickness colour-coded red. Such 3D thickness-models enabled initial identification of the dividing membrane (DivM) that separates the tracheal branches. The thinness of the DivM was subsequently statistically compared against four other structures (two membranes and two controls) using the same 3D analysis method here visualised. Both this 3D model and the segmentations used for the statistical comparison (n=12) were scanned with following specifications: an exposure time of 1325ms, 0.2° rotation steps for 180°, a frame averaging of 2, a current of 100 µA, and without a metal filter. In distinction, the specimen here visualised was scanned with the following specific parameters: 30 kV X-ray tube voltage and a resolution of 0.88 µm voxels. [Latham B, Reid A, Jackson-Camargo JC, Williams JA, Windmill JFC. Coupled membranes: A mechanism of frequency filtering and transmission in the field cricket ear evidenced by micro-computed tomography, laser Doppler vibrometry, and finite element analysis. J. R. Soc. Interface.]

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