Thermal performance curves showing the relationship between productivity (A) and temperature in individual derived lineages of Bacillus thuringiensis (Bt) and Bacillus mycoides (Bm) selected at 30°C from Constraints in temperature adaptation reinforce differences in thermal niche between mesophilic and psychrotolerant Bacillus cereus group species

Supplemental figure 1: The relationship between productivity (A) and temperature in individual derived lineages of Bacillus thuringiensis (Bt) and Bacillus mycoides (Bm) selected at 30°C (thermal performance curves, TPCs). TPCs were quantified by the Sharpe-Schoolfield model as used previously and shown here using the red lines with residual bootstrapped confidence intervals. These were used to extract the following parameters; the optimum growth temperature, Topt; the maximum growth rate/productivity, Bpk/Ppk, and the operational thermal niche width, Wop. Dots indicate productivity and growth rate values for growth curves of replicate cultures at each temperature. Note that Lineage 2 of B. thuringiensis selected at 30°C has a high peak of productivity at 39°C; this is believed to be experimental error, so this lineage was not included in the productivity analysis.