Glycosylation system investigation, Terra et al. Table S1 ;Glycosylation system investigation, Terra et al. Table S2;Glycosylation system investigation, Terra et al. Figure S1;Glycosylation system investigation, Terra et al. Figure S2 from The N-linking glycosylation system from Actinobacillus pleuropneumoniae is required for adhesion and has potential use in glycoengineering

Strains and plasmids used in this study; Primers used in this study for reverse transcriptase analysis of RNA extracts from A. pleuropneumoniae HS143, complementation of ngt and agt mutants and construction of A. pleuropneumoniae deletion mutants; Expression analyses of ngt and sodC. Reverse transcriptase PCR results of RNA extracted from A. pleuropneumoniae serotype 15 at various time points and in various mutants. 1, 1.5 hr cDNA template; 2, 1.5 hr RNA template; 3, 3.0 hr cDNA template; 4, 3.0 hr RNA; 5, 5.0 hr cDNA template; 6, 5.0 hr RNA template; 7, Δngt overnight culture cDNA template; 8, Δngt overnight culture RNA template; 9, Δngt complemented with pMKExpressNGT overnight culture cDNA template; 10, Δngt complemented with pMKExpressNGT overnight culture RNA template; 11, genomic DNA template (positive PCR control).; Predicted transcriptional promoter and Rho-independent terminators identified by bioinformatics analysis. V. Solovyev, A Salamov (2011) Automatic Annotation of Microbial Genomes and Metagenomic Sequences. In Metagenomics and its Applications in Agriculture, Biomedicine and Environmental Studies (Ed. R.W. Li), Nova Science Publishers, p. 61-78