Figure S2 from Human transposon insertion profiling by sequencing (TIPseq) to map LINE-1 insertions in single cells
journal contributionposted on 24.12.2019, 16:16 by Wilson McKerrow, Zuojian Tang, Jared P. Steranka, Lindsay M. Payer, Jef D. Boeke, David Keefe, David Fenyö, Kathleen H. Burns, Chunhong Liu
Comparison of sensitivity for all gold standard L1Hs and only heterozygous non-reference insertions. Black, bulk DNA TIPseq; red, MDA whole genome amplification followed by restriction enzyme digestion and ligation with vectorette adaptors (MDA-D); orange, MDA whole genome amplification followed by end repair, dA tailing and ligation with dT vectorette adaptor (MDA-T); dark blue, MALBAC whole genome amplification followed by ligation with dT vectorette adaptor (MALBAC-T). Diamond, bulk DNA TIPseq; circle, whole genome amplification using random hexamers only (R); square, whole genome amplification using random hexamers and L1 primer (RL).