Fig. S1. Rough spot tests identified 62 candidate strains, the ts phenotype of which could be rescued by 0.1 µg/mL rapamycin.; Fig. S2. Manual spot tests of 62 selected candidates identified 45 strains with clear rescue at 36ºC (underlined).; Fig. S3. 19 strains with cut1 mutations showed a ts phenotype rescued by rapamycin.; Fig. S4. Rapamycin rescued the ts phenotype of sty1-989.;

Fig. S1. Rough spot tests identified 62 candidate strains, the ts phenotype of which could be rescued by 0.1 µg/mL rapamycin. Left side: plate images of spot tests and candidates are shown with red squares. Right side: plate layouts of ts strains. Orange-shaded numbers indicate selected candidates.; Fig. S2. Manual spot tests of 62 selected candidates identified 45 strains with clear rescue at 36ºC (underlined).; Fig. S3. 19 strains with cut1 mutations showed a ts phenotype rescued by rapamycin. (A) Plate images of spot tests are shown. Red- and orange-lettered strains were rescued at 36ºC and 33ºC, respectively. (B) Mutation sites and locations in Cut1protein are shown for these 19 strains. The majority of mutations localized around leucine repeats and a peptidase domain.; Fig. S4. Rapamycin rescued the ts phenotype of sty1-989. (A) Cell number increment of sty1-989 in EMM2 liquid medium with or without 200 nM rapamycin at 26ºC or 36ºC was plotted in a time course. (B) DAPI images of WT and sty1-989 cells at 26ºC and 36ºC in EMM2 media with or without 200 nM rapamycin.