Supplementary Figures and Tables from Toxicity and taste: unequal chemical defences in a mimicry ring

Figure S1: Representative photographs of the non-red spot species: A) Ceratosoma amoenum, B) Chromodoris kuiteri, C) Chromodoris lochi, D) Chromodoris elisabethina, E) Doriprismatica atromarginata, F) Goniobranchus decorus, G) Goniobranchus geometricus, H) Hypselodoris jacksoni, I) Hypselodoris obscura, J) Hypselodoris tryoni, K) Hypselodoris whitei, L) Risbecia godeffroyana; Figure S2: a) Toxicity assay: LD50 values based on mortality of Brine shrimp, Artemia sp. b) Anti-feedant assay. ED50 values based on rejection of pellets by Palaemon shrimp, Palaemon serenus Y-axis indicates absolute concentrations rather than proportion of natural concentration (Figure 4). Cross indicates no response (nr) observed when tested at the highest concentration. Open circle indicates that a response was observed but not significant enough to calculate an ED50 value.; Figure S3: ED50 and LD50 values are plotted for 6 species. The graph is separated into four zones, which coincide with relative toxicity and/or palatability of the species within.; Figure S4: Dose response curves for a) brine shrimp assays and b) palaemon shrimp assays.; Table S1: Specimen collection information.; Table S2: Species and data used to construct the phylogeny. Genbank accession numbers are listed for COI and 16S genes along with specimen voucher numbers. Outgroups are shown in bold. * indicates that for these species new sequences were generated as part of this study.; Table S3: Colour pattern characteristics for each species used in NMDS plots. Zero values are generated for hue if the distance from the achromatic point was very low and hence the angle and direction from the achromatic point unreliable. When the pattern was complex, the dominant pattern used to calculate the measurements are named below the values.; Table S4: Chemical compounds found in each species of nudibranch.