Supplementary Figure 2. Immobile mutant worms do not alter their morphology when grown in liquid, but their progeny are longer. from Inter-generational consequences for growing C. elegans in liquid

(A) Representative photographs of plate- and liquid- grown wild type and unc-119 adult worms. (B) Quantification of the length of the parental unc-119 mutants grown either on plates or in liquid. The measured length was normalized to the corresponding control worms. Data from three independent biological repeats are presented (N>80). ****p-value<0.0001, Sidak's multiple comparisons test. (C) Plate-grown progeny of immobile unc-119 mutant worms are longer than plate grown controls. The length of F1 plate-grown progeny of liquid-grown worms and progeny of plate-grown controls are shown. The measured length was normalized to the corresponding control worms. Data from three independent biological repeats are presented (N>130 worms per group). The p-value was determined using Two-way ANOVA with the two categorical variables: A. condition (growth in liquid or on plates) and B. biological repeat. The biological condition factor is significant (p-value<0.0001). The biological repeat factor and interaction effect are also significant (p=0.0024 for both). The biological condition factor explains more variance (2.76%) than the biological repeat or the interaction effect factors (1%). ****p-value<0.0001, **p-value= 0.0027, Sidak's multiple comparisons test.