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Serine-38 of Ana2 is phosphorylated in vitro by Plk4 as identified by mass-spectrometry. from Two-step phosphorylation of Ana2 by Plk4 is required for the sequential loading of Ana2 and Sas6 to initiate procentriole formation

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posted on 08.12.2017 by Nikola S. Dzhindzhev, George Tzolovsky, Zoltan Lipinszki, Mohammed Abdelaziz, Janus Debski, Michal Dadlez, David M. Glover
Supplementary Figure S2. Serine-38 of Ana2 is phosphorylated in vitro by Plk4 as identified by mass-spectrometry. Phospho-Mascot-interpreted fragmentation spectra of the peptide 13-LAPRP…EV (phospho-S-38) ILFG…SPR-65 with phosphorylated serine at the position 38 (highlighted red). Labelled peaks correspond to fragment ions. Despite the relatively long peptide, the fragmentation pattern unambiguously identifies the phosphorylation site.

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