rspb20180891_si_002.pdf (2.86 MB)

Figure S1. Experimental design from Indirect ecological effects interact with community genetic effects in a host–parasite system and dramatically reduce parasite burden

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journal contribution
posted on 17.08.2018 by Mouhammad Shadi Khudr, Samuel Alexander Purkiss, Reinmar Hager
The schematic diagram illustrates the concept and the design of the experiment. Eight clonal aphids from two genotypes (G1 and G2) were placed in 12 different microcosms, each containing four cabbage plants of either red cabbage (R), savoy cabbage (S) or two of each (RS). Six microcosm types were grown on soil that contained 15% onion-trained soil (with inoculum), while six others served as a control (without inoculum). Each of the 12 microcosm types was replicated five times. The community genetic effects (CG) comprised the genetic variability within species for cabbage (two cultivars) and aphids (two clones/genotypes). The indirect ecological effect (IEE) was induced via a plant-soil feedback (PSF) mechanism resulting from the inoculation with a sample of soil-trained with insect repellent plant (onion, Allium cepa). CG and IEE are envisioned to interact and shape aphid reproductive success (population size).