siRNAs for E6 and E7 silencing are functional from Upregulation of PIR gene expression induced by human papillomavirus E6 and E7 in epithelial oral and cervical cells

(a, b) The usefulness of siRNAs and scrambled for HPV-16 E6 and E7 silencing was evaluated by RT-qPCR. (c) Immunofluorescence by confocal microscopy for HPV-16 E7 oncoprotein in Ca Ski cells transiently transfected with siRNA against E7 or scrambled. Results are representative of three independent experiments.