siRNA for PIR silencing is functional from Upregulation of PIR gene expression induced by human papillomavirus E6 and E7 in epithelial oral and cervical cells

Ca Ski cells were transiently transfected with a siRNA or scrambled for PIR silencing and the levels of PIR transcripts or pirin protein were evaluated by (a) RT-qPCR, (b) western blotting and (c) immunofluorescence. Results are representative of three independent experiments.