rspb20172784_si_003.jpg (330.98 kB)
Supplemental Fig. 1 from To eat or not to eat: ontogeny of hypothalamic feeding controls and a role for leptin in modulating life-history transition in amphibian tadpoles
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posted on 2018-03-19, 14:28 authored by Melissa Cui Bender, Caroline Hu, Chris Pelletier, Robert J. DenverDorsal view of Xenopus brain showing the three regions dissected for gene expression analysis by RTqPCR (anatomical drawing from Tuinhof et al.) [1]. B. Western blot of rxLeptin using rabbit anti-rxLeptin serum. We resolved 100 ng of rxLeptin by 10% PAGE before transfer to nitrocellulose for immunoblotting. The rxLeptin, which has poly-histidine and V5 tags, has an expected molecular weight of 21.6 kD.