Video supplement V2 from A selective fungal transport organ (mycangium) maintains coarse phylogenetic congruence between fungus-farming ambrosia beetles and their symbionts

Extraction of Xylosandrus compactus mycangium contents. Beetles were mounted live on ultrafine pins. Fungal masses were extracted from mycangia by applying gentle pressure to the scutellum. This technique allows a sanitary removal of the complete living fungal mass for quantification of viable spores by serial dilution and culture. Mycangia that did not contain a fungal mass were verified by complete eversion of the empty mycangium.