Supplementary Figures 1 - 3 from Inter-generational consequences for growing C. elegans in liquid.

Figure S1. F1 progeny of liquid grown worms are longer irrespectively of the concentration in which their liquid-grown parents were grown. The normalized lengths of F1 progeny of worms grown in liquid in different concentrations (x-axis) and progeny of plate-grown controls are presented. The measured length is normalized to the corresponding control progeny of plate-grown worms. ****p-value <0.0001. One-way ANOVA, Sidak's multiple comparisons test. Error bars represent standard deviations.; Figure S2. Immobile mutant worms do not alter their morphology when grown in liquid, but their progeny are longer. (a) Representative photographs of plate- and liquid- grown wild type and unc-119 adult worms. (b) Quantification of the length of the parental unc-119 mutants grown either on plates or in liquid. The measured length was normalized to the corresponding control worms. Data from three independent biological repeats are presented (N>80). ****p-value<0.0001, Sidak's multiple comparisons test. (c) Plate-grown progeny of immobile unc-119 mutant worms are longer than plate grown controls. The length of F1 plate-grown progeny of liquid-grown worms and progeny of plate-grown controls are shown. The measured length was normalized to the corresponding control worms. Data from three independent biological repeats are presented (N>130 worms per group). The p-value was determined using Two-way ANOVA with the two categorical variables: A. condition (growth in liquid or on plates) and B. biological repeat. The biological condition factor is significant (p-value<0.0001). The biological repeat factor and interaction effect are also significant (p=0.0024 for both). The biological condition factor explains more variance (2.76%) than the biological repeat or the interaction effect factors (1%). ****p-value<0.0001, **p-value= 0.0027, Sidak's multiple comparisons test.; Figure S3. Linear regression analysis of growth rates of progeny of liquid-grown and control worms. Linear regression analysis of the length of progeny of liquid-grown worms and control worms at different time-points during the worm's development. While the intercepts are significantly different (p=0.022), the rate of growth, or regression coefficients are identical (p=0.7806). Error bars represent standard deviations.