Supplemental Fig. 4 from To eat or not to eat: ontogeny of hypothalamic feeding controls and a role for leptin in modulating life-history transition in amphibian tadpoles
2018-03-19T14:28:18Z (GMT) by
Functional leptin signaling in tadpole pituitary increased during metamorphosis. We investigated changes in functional leptin signaling in the tadpole pituitary gland brain during metamorphosis by analyzing rxLeptin-induced phospho-STAT3 immunoreactivity (pSTAT3-ir). A. The arrow on the anatomical drawing of the dorsal view of Xenopus brain shows the location of the transverse section shown at right, after Tuinhof et al. . Abbreviations: tect – optic tectum, tegm – mesencephalic tegmentum, TP – posterior tuberculum, pd – pars distalis, pi – pars intermedia, pn – pars nervosa. B. Tadpoles were given i.c.v. injections of 0.6% saline or rxLeptin (20 ng/g*BW) and killed one hr later for immunohistochemistry for pSTAT3-ir (see Supplemental Methods). The scale bar equals 50 m and applies to all photomicrographs in the panels. C. Changes in mean nuclei count of pSTAT3-ir cells in the tadpole pars distalis during metamorphosis. We counted the number of pSTAT3-ir nuclei following i.c.v. injection of rxLeptin as described in the Materials and Methods. Shown are the means±SEM (n=3-4/developmental stage). We saw statistically significant changes in pSTAT3-ir in the pars distalis during metamorphosis (F(4,12)=10.211, p=0.001; ANOVA). Means with the same letter are not significantly different (p<0.05; Fisher’s LSD test).