Supplemental Fig. 1 from To eat or not to eat: ontogeny of hypothalamic feeding controls and a role for leptin in modulating life-history transition in amphibian tadpoles

Dorsal view of Xenopus brain showing the three regions dissected for gene expression analysis by RTqPCR (anatomical drawing from Tuinhof et al.) [1]. B. Western blot of rxLeptin using rabbit anti-rxLeptin serum. We resolved 100 ng of rxLeptin by 10% PAGE before transfer to nitrocellulose for immunoblotting. The rxLeptin, which has poly-histidine and V5 tags, has an expected molecular weight of 21.6 kD.