rsob170247_si_002.pdf (67.29 kB)
Serine-38 of Ana2 is phosphorylated in vitro by Plk4 as identified by mass-spectrometry. from Two-step phosphorylation of Ana2 by Plk4 is required for the sequential loading of Ana2 and Sas6 to initiate procentriole formation
journal contribution
posted on 2017-12-08, 11:42 authored by Nikola S. Dzhindzhev, George Tzolovsky, Zoltan Lipinszki, Mohammed Abdelaziz, Janus Debski, Michal Dadlez, David M. GloverSupplementary Figure S2. Serine-38 of Ana2 is phosphorylated in vitro by Plk4 as identified by mass-spectrometry. Phospho-Mascot-interpreted fragmentation spectra of the peptide 13-LAPRP…EV (phospho-S-38) ILFG…SPR-65 with phosphorylated serine at the position 38 (highlighted red). Labelled peaks correspond to fragment ions. Despite the relatively long peptide, the fragmentation pattern unambiguously identifies the phosphorylation site.