ROS induces ZAG oligomerization from Zinc-α2-glycoprotein is an inhibitor of amine oxidase copper-containing 3

2019-07-22T08:12:19Z (GMT) by Matthias Romauch
Supplemental Fig. 3 A and B, WB: Wt ZAG and its glycomutants were overexpressed in Expi293F cells and affinity purified using their GST-tags. ZAG (after GST removal) was incubated with and without recombinant AOC3, in the presence of H2O2 (A) or benzylamine (B). Samples were separated by non-reducing SDS-PAGE and proteins were probed using anti-ZAG antibody. C, WB: Plasma of a C57Bl6 wt mouse. Proteins were separated by non-reducing SDS-PAGE and proteins were probed using anti-ZAG antibody. Asterisks indicate a high molecular weight protein complex.