Phylogenetic analysis and activity of Mn-SOD. from Enhancement of oxidative stress contributes to increased pathogenicity of the invasive pine wood nematode

(A) The phylogenetic analysis of Mn-SOD amino acids from B. xylophilus and other organisms. The sequences and NCBI accession number used are listed as follows: NP_038699.2, Mus musculus; NP_956270.1, Danio rerio; NP_492290.1, Caenorhabditis elegans; NP_187703.1, Arabidopsis thaliana; WP_032292902.1, Escherichia coli; NP_476925.1, Drosophila melanogaster; NP_001055195.1, Oryza sativa; XP_003115144.1, Caenorhabditis remanei; NP_011872.1, Saccharomyces cerevisiae; CDJ98014.1, Haemonchus contortus; XP_013295293.1, Necator americanus; KIH60312.1, Ancylostoma duodenale. (B) The plotted curve depicts the rate of inhibition as a function of Mn-SOD protein concentration. The inhibition rate~ 50% corresponds to one unit of enzyme activity.