Movie S3 from Substrate protein dependence of GroEL–GroES interaction cycle revealed by high-speed atomic force microscopy imaging

HS-AFM movie of GroEL−GroES interaction during steady-state ATPase reaction cycle in the absence of substrate protein. D490C GroEL biotinylated at Cys490 was immobilized on a surface of tamavidin 2-LPI 2D crystals in a side-on orientation, while GroES was free in the bulk solution. The images were acquired at 4.35 fps for an area of 64 × 80 nm2. Z-scale, 14.5 nm.