rstb20170180_si_001.mov (458.32 kB)
Movie S1 from Substrate protein dependence of GroEL–GroES interaction cycle revealed by high-speed atomic force microscopy imaging
Version 2 2020-10-16, 10:20
Version 1 2018-03-31, 11:15
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posted on 2018-03-31, 11:15 authored by Daisuke Noshiro, Toshio AndoHS-AFM movie of GroEL−GroES interaction during steady-state ATPase reaction cycle in the presence of DM-MBP. D490C GroEL biotinylated at Cys490 was immobilized on a surface of tamavidin 2-LPI 2D crystals in a side-on orientation, while GroES and denature DM-MBP were free in the bulk solution. The images were acquired at 4.35 fps for an area of 64 × 80 nm2. Z-scale, 14.5 nm.