Figure S5. Rab1 is required to recruit Garz to the Golgi membranes. from Rab1 interacts with GOLPH3 and controls Golgi structure and contractile ring constriction during cytokinesis in <i>Drosophila melanogaster</i>

(a) Interphase spermatocytes from wild type and omt/Df stained for Garz, Tubulin and DNA. (N=32 wild type spermatocytes, N=36 omt/Df mutant spermatocytes; the cells examined were from four independent experiments). Scale bar, 10 μm. (b) Co-IP of Garz with RFP-Rab1. Protein extracts from testes expressing either RFP-Rab1 or RFP (as a control) proteins were immunoprecipitated with anti-RFP (i.e RFP-trap beads) and immunoblotted for RFP and Garz. 2% of the total lysates (Input), and 1/3 of the immunoprecipitates were loaded and probed with the indicated antibody. Molecular masses are in kilodalton. (c) Quantification of Garz protein Co-immunoprecipitated from testis protein extracts expressing either RFP, or RFP-Rab1. The protein band intensities were obtained from three independent experiments.



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