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Figure S5. Rab1 is required to recruit Garz to the Golgi membranes. from Rab1 interacts with GOLPH3 and controls Golgi structure and contractile ring constriction during cytokinesis in Drosophila melanogaster

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posted on 03.01.2017 by Stefano Sechi, Anna Frappaolo, Roberta Fraschini, Luisa Capalbo, Marco Gottardo, Giorgio Belloni, David M. Glover, Alan Wainman, Maria Grazia Giansanti
(a) Interphase spermatocytes from wild type and omt/Df stained for Garz, Tubulin and DNA. (N=32 wild type spermatocytes, N=36 omt/Df mutant spermatocytes; the cells examined were from four independent experiments). Scale bar, 10 μm. (b) Co-IP of Garz with RFP-Rab1. Protein extracts from testes expressing either RFP-Rab1 or RFP (as a control) proteins were immunoprecipitated with anti-RFP (i.e RFP-trap beads) and immunoblotted for RFP and Garz. 2% of the total lysates (Input), and 1/3 of the immunoprecipitates were loaded and probed with the indicated antibody. Molecular masses are in kilodalton. (c) Quantification of Garz protein Co-immunoprecipitated from testis protein extracts expressing either RFP, or RFP-Rab1. The protein band intensities were obtained from three independent experiments.

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