Figure S4 from Ex vivo gut culture for studying differentiation and migration of small intestinal epithelial cells

Effects of Wnt, BMP and Notch signaling on intestinal differentiation of ex vivo guts. Small intestine from E13.5 embryos were isolated and cultured in medium containing 100 nM LDN-193189 (BMP signaling inhibitor; a), 10 μM DAPT (Notch signaling inhibitor; b), or 100 ng/ml Wnt 3a plus 500 ng/ml R-spondin1 (Wnt signaling activator; c). Relative mRNA expressions were tested using RT-qPCR. Data are represented as mean ± SEM. N=4. *: P < 0.05 versus CON. **: P < 0.01 versus CON.