Figure S3 from Ex vivo gut culture for studying differentiation and migration of small intestinal epithelial cells

Interstitial cells of Cajal and Foxl1-expressing mesenchymal cells in ex vivo guts.Ex vivo guts were cultured for 7 days. (a-b) Relative C-kit and Foxl1 mRNA in ex vivo guts (EVG). mRNA from intestine and fat tissue of adult mice was used as a positive control (PC) and negative control (NC), respectively. (c) The relative contraction frequencies of ex vivo guts were treated with botulinum neurotoxin for 0, 6, 12 and 24h. Data are represented as mean ± SEM. Each ex vivo gut from a dedicated mouse was view as an experiment unit, n=5. *: P < 0.05 versus 0h. **: P < 0.01 versus 0h.