Figure S2 from Ex vivo gut culture for studying differentiation and migration of small intestinal epithelial cells

Representative images of negative controls in immunofluorescent staining. Sections were permeabilized, incubated with 5% goat serum overnight at 4°C, and incubated with respective fluorescent secondary antibodies for 2h at room temperature. Immunofluorescence was imaged using a fluorescence microscope. Scale bar is 200 μm.