Figure S2 from Variation in defence strategies in the metal hyperaccumulator plant Noccaea caerulescens is indicative of synergies and trade-offs between forms of defence

Figure S2: Superoxide production in response to Psm or MgCl2 inoculation in N. caerulescens plants of the four populations, and in A. thaliana. N. caerulescens plants were treated with 0.04-300 micromolar zinc. Three leaves of each of six plants were infiltrated with P. syringae pv. maculicola M4 at 107 cfu/ml in 10 mM MgCl2, with 10 mM MgCl2, or left untreated. For A. thaliana, Psm was inoculated instead at 105 cfu/ml. After 1, 2, or 6 h, leaves were excised and stained overnight in 0.01% (w/v) NBT solution before bleaching and image analysis. ANOVAs were used to test for significant differences in percentage staining between different inoculation treatments at each zinc concentration and for Arabidopsis. Bonferroni simultaneous comparisons were carried out where significance was found (α = 0.05); means which are not significantly different are marked with the same letter. Results shown here represent the mean of two independent experiments. Error bars indicate SE.