Figure S2 from Variation in defence strategies in the metal hyperaccumulator plant <i>Noccaea caerulescens</i> is indicative of synergies and trade-offs between forms of defence

<b>Figure S2: Superoxide production in response to <i>Psm</i> or MgCl<sub>2</sub> inoculation in <i>N. caerulescens</i> plants of the four populations, and in <i>A. thaliana</i>.</b> <i>N. caerulescens</i> plants were treated with 0.04-300 micromolar zinc. Three leaves of each of six plants were infiltrated with <i>P. syringae</i> pv. maculicola M4 at 10<sup>7</sup> cfu/ml in 10 mM MgCl<sub>2</sub>, with 10 mM MgCl<sub>2</sub>, or left untreated. For <i>A. thaliana</i>, <i>Psm</i> was inoculated instead at 10<sup>5</sup> cfu/ml. After 1, 2, or 6 h, leaves were excised and stained overnight in 0.01% (w/v) NBT solution before bleaching and image analysis. ANOVAs were used to test for significant differences in percentage staining between different inoculation treatments at each zinc concentration and for <i>Arabidopsis</i>. Bonferroni simultaneous comparisons were carried out where significance was found (α = 0.05); means which are not significantly different are marked with the same letter. Results shown here represent the mean of two independent experiments. Error bars indicate SE.