Figure S1 from Ex vivo gut culture for studying differentiation and migration of small intestinal epithelial cells

Cell viability, alkaline phosphatase activity and Gata4 expression of ex vivo guts. (a-g) Small intestine from E13.5 embryos were isolated and cultured to form ex vivo guts, and stained with trypan blue at 3d (a), 5d (b), 7d (c), and 10d (d). (e-f) Magnified image of a selected area in panel c-d. Scale bars are 400 μm in (a-d), and 200 μm in (e-f). (g) Alkaline phosphatase activity of ex vivo guts at 0d, 3d, 5d, 7d, and 10d. Mouse tails from E13.5 embryo were used as the negative control (NC). (f) Ex vivo guts from embryonic distal or proximal small intestine were cultured at 7d. Relative mRNA expression of Gata4 was tested using RT-qPCR. Data are represented as mean ± SEM. N=4. *: P < 0.05 versus distal small intestine. **: P < 0.01 versus 0d.