Figure S1 from Clonal yeast biofilms can reap competitive advantages through cell differentiation without being obligatorily multicellular

Morphology of natural S. cerevisiae isolates. Inoculations were always 500 cells in the center of semisolid complex medium plates (9 cm diameter, 0.3% agar, YPD), which were grown for seven days at room temperature as previously described [21]. Large structured colonies (YJM653; DBVPG6861; YPS163; Y55, CLIB326; YJM678) were biofilm formers, whereas all others were wrinkled small colonies (DBVPG46651, YJM981) and smooth small colonies that did not form biofilms. NC-02, Y9, Y10 formed smaller slightly structured colonies and were recorded as smooth.