Fig S3 from Role of long-chain acyl-CoAs in the regulation of mycolic acid biosynthesis in mycobacteria

Effect of C26-CoA on the formation of C20-CoA/ His6-MabRMT /PfasII complex. His6-MabRMT was preincubated during 5 min with C26-CoA in the presence of poly-dIdC, prior to the addition of C20-CoA and 5 min later, PfasII probe was added (last 2 lanes). The His6-MabRMT-DNA complex formation was also assayed with the individual addition of different concentrations of C20-CoA (lanes 3-6) or C26-CoA (lanes 7-10), as controls of the experiment.