Fig S3 from Role of long-chain acyl-CoAs in the regulation of mycolic acid biosynthesis in mycobacteria

Effect of C₂₆-CoA on the formation of C₂₀-CoA/ His₆-MabR_MT /PfasII complex. His₆-MabR_MT was preincubated during 5 min with C₂₆-CoA in the presence of poly-dIdC, prior to the addition of C₂₀-CoA and 5 min later, PfasII probe was added (last 2 lanes). The His₆-MabR_MT-DNA complex formation was also assayed with the individual addition of different concentrations of C₂₀-CoA (lanes 3-6) or C₂₆-CoA (lanes 7-10), as controls of the experiment.