Supplementary Material from Bacterial ageing in the absence of external stressors ŁapińskaUrsula GloverGeorgina Capilla-LasherasPablo YoungAndrew J. PagliaraStefano 2019 This document contains supporting data regarding the optimisation of E. coli staining with thioflavin T to investigate the formation of misfolded protein aggregates and regarding the uptake of the fluorescent glucose analogue 2-NBDG in single E. coli placed at different positions of the mother machine channels.