Supplementary materials and methods from Convergent evolution of cytochrome P450s underlies independent origins of keto-carotenoid pigmentation in animals Nicky Wybouw Andre H. Kurlovs Robert Greenhalgh Astrid Bryon Olivia Kosterlitz Yuki Manabe Masahiro Osakabe John Vontas Richard M. Clark Thomas Van Leeuwen 10.6084/m9.figshare.8362082.v1 https://rs.figshare.com/articles/journal_contribution/Supplementary_materials_and_methods_from_Convergent_evolution_of_cytochrome_P450s_underlies_independent_origins_of_keto-carotenoid_pigmentation_in_animals/8362082 Keto-carotenoids contribute to many important traits in animals, including vision and coloration. In a great number of animal species, keto-carotenoids are endogenously produced from carotenoids by carotenoid ketolases. Despite the ubiquity and functional importance of keto-carotenoids in animals, the underlying genetic architectures of their production have remained enigmatic. The body and eye colorations of spider mites (Arthropoda: Chelicerata) are determined by β-carotene and keto-carotenoid derivatives. Here, we focus on a carotenoid pigment mutant of the spider mite <i>Tetranychus kanzawai</i> that<i>,</i> as shown by chromatography, lost the ability to produce keto-carotenoids. We employed bulked segregant analysis and linked the causal locus to a single narrow genomic interval. The causal mutation was fine-mapped to a minimal candidate region that held only one complete gene, the cytochrome P450 monooxygenase <i>CYP384A1</i>, of the CYP3 clan. Using a number of genomic approaches, we revealed that an inactivating deletion in the fourth exon of <i>CYP384A1</i> caused the aberrant pigmentation. Phylogenetic analysis indicated that CYP384A1 is orthologous across mite species of the ancient Trombidiformes order where carotenoids typify eye and body coloration, suggesting a deeply conserved function of CYP384A1 as a carotenoid ketolase. Previously, <i>CYP2J19,</i> a cytochrome P450 of the CYP2 clan, has been identified as a carotenoid ketolase in birds and turtles. Our study shows that selection for endogenous production of keto-carotenoids led to convergent evolution, whereby cytochrome P450s were independently co-opted in vertebrate and invertebrate animal lineages. 2019-06-29 12:11:20 carotenoid ketolase convergent evolution keto-carotenoids lemon CYP384A1