Supplementary Material 9 - Code from Heightened condition dependence of the sexual transcriptome as a function of genetic quality in <i>Drosophila melanogaster</i> head tissue Antonino Malacrinò Christopher M. Kimber Martin Brengdahl Urban Friberg 10.6084/m9.figshare.8280917.v1 https://rs.figshare.com/articles/dataset/Supplementary_Material_9_-_Code_from_Heightened_condition_dependence_of_the_sexual_transcriptome_as_a_function_of_genetic_quality_in_i_Drosophila_melanogaster_i_head_tissue/8280917 Theory suggests sexual traits should show heightened condition dependent expression. This prediction has been tested extensively in experiments where condition has been manipulated through environmental quality. Condition dependence as a function of genetic quality has, however, only rarely been addressed, despite its central importance in evolutionary theory. To address the effect of genetic quality on expression of sexual and non-sexual traits, we here compare gene expression in <i>Drosophila melanogaster</i> head tissue between flies with intact genomes (high condition) and flies carrying a major deleterious mutation (low condition). We find that sex-biased genes show heightened condition dependent expression in both sexes, and that expression in low condition males and females regresses towards a more similar expression profile. As predicted, sex-biased expression was more sensitive to condition in males compared to females, but surprisingly female-biased, rather than male-biased, genes show the higher sensitivity to condition in both sexes. Our results thus support the fundamental predictions of the theory of condition dependence when condition is a function of genetic quality. 2019-06-15 12:40:22 condition dependence Drosophila melanogaster gene expression genetic quality sexual dimorphism