10.6084/m9.figshare.7598993.v1 Itamar Lev Itamar Lev Roberta Bril Roberta Bril Yunan Liu Yunan Liu Lucila Inés Ceré Lucila Inés Ceré Oded Rechavi Oded Rechavi Supplementary Figure 2. Immobile mutant worms do not alter their morphology when grown in liquid, but their progeny are longer. from Inter-generational consequences for growing <i>C. elegans</i> in liquid The Royal Society 2019 trans-generational inter-generational liquid inheritance epigenetics C. elegans 2019-01-17 07:47:14 Journal contribution https://rs.figshare.com/articles/journal_contribution/Supplementary_Figure_2_Immobile_mutant_worms_do_not_alter_their_morphology_when_grown_in_liquid_but_their_progeny_are_longer_from_Inter-generational_consequences_for_growing_i_C_elegans_i_in_liquid/7598993 (A) Representative photographs of plate- and liquid- grown wild type and unc-119 adult worms. (B) Quantification of the length of the parental unc-119 mutants grown either on plates or in liquid. The measured length was normalized to the corresponding control worms. Data from three independent biological repeats are presented (N>80). ****p-value<0.0001, Sidak's multiple comparisons test. (C) Plate-grown progeny of immobile unc-119 mutant worms are longer than plate grown controls. The length of F1 plate-grown progeny of liquid-grown worms and progeny of plate-grown controls are shown. The measured length was normalized to the corresponding control worms. Data from three independent biological repeats are presented (N>130 worms per group). The p-value was determined using Two-way ANOVA with the two categorical variables: A. condition (growth in liquid or on plates) and B. biological repeat. The biological condition factor is significant (p-value<0.0001). The biological repeat factor and interaction effect are also significant (p=0.0024 for both). The biological condition factor explains more variance (2.76%) than the biological repeat or the interaction effect factors (1%). ****p-value<0.0001, **p-value= 0.0027, Sidak's multiple comparisons test.