10.6084/m9.figshare.7570838.v1 Helen N. Fones Helen N. Fones Gail M. Preston Gail M. Preston J. Andrew J. Andrew C. Smith C. Smith Figure S2 from Variation in defence strategies in the metal hyperaccumulator plant <i>Noccaea caerulescens</i> is indicative of synergies and trade-offs between forms of defence The Royal Society 2019 Noccaea hyperaccumulator pseudomonas elemental defence zinc cell death glucosinolates 2019-01-10 08:46:56 Journal contribution https://rs.figshare.com/articles/journal_contribution/Figure_S2_from_Variation_in_defence_strategies_in_the_metal_hyperaccumulator_plant_i_Noccaea_caerulescens_i_is_indicative_of_synergies_and_trade-offs_between_forms_of_defence/7570838 <b>Figure S2: Superoxide production in response to <i>Psm</i> or MgCl<sub>2</sub> inoculation in <i>N. caerulescens</i> plants of the four populations, and in <i>A. thaliana</i>.</b> <i>N. caerulescens</i> plants were treated with 0.04-300 micromolar zinc. Three leaves of each of six plants were infiltrated with <i>P. syringae</i> pv. maculicola M4 at 10<sup>7</sup> cfu/ml in 10 mM MgCl<sub>2</sub>, with 10 mM MgCl<sub>2</sub>, or left untreated. For <i>A. thaliana</i>, <i>Psm</i> was inoculated instead at 10<sup>5</sup> cfu/ml. After 1, 2, or 6 h, leaves were excised and stained overnight in 0.01% (w/v) NBT solution before bleaching and image analysis. ANOVAs were used to test for significant differences in percentage staining between different inoculation treatments at each zinc concentration and for <i>Arabidopsis</i>. Bonferroni simultaneous comparisons were carried out where significance was found (α = 0.05); means which are not significantly different are marked with the same letter. Results shown here represent the mean of two independent experiments. Error bars indicate SE.