Figure S1 from Variation in defence strategies in the metal hyperaccumulator plant <i>Noccaea caerulescens</i> is indicative of synergies and trade-offs between forms of defence Helen N. Fones Gail M. Preston J. Andrew C. Smith 10.6084/m9.figshare.7570832.v1 https://rs.figshare.com/articles/journal_contribution/Figure_S1_from_Variation_in_defence_strategies_in_the_metal_hyperaccumulator_plant_i_Noccaea_caerulescens_i_is_indicative_of_synergies_and_trade-offs_between_forms_of_defence/7570832 <b>Figure S1: Hydrogen peroxide production in response to <i>Psm</i> or MgCl<sub>2</sub> inoculation in <i>N. caerulescens</i> plants of the four populations, and in <i>A. thaliana</i>.</b> <i>N. caerulescens</i> plants were treated with 0.04-300 micromolar zinc. Three leaves of each of six plants were infiltrated with <i>P. syringae</i> pv. maculicola M4 at 10<sup>7</sup> cfu/ml in 10 mM MgCl<sub>2</sub>, with 10 mM MgCl<sub>2</sub>, or left untreated. For <i>A. thaliana</i>, <i>Psm</i> was inoculated instead at 10<sup>5</sup> cfu/ml. After 1, 2, or 6 h, leaves were excised and stained overnight in 0.01% (w/v) DAB solution before bleaching and image analysis. ANOVAs were used to test for significant differences in percentage staining between different inoculation treatments at each zinc concentration and for <i>Arabidopsis</i>. Bonferroni simultaneous comparisons were carried out where significance was found (α = 0.05); means which are not significantly different are marked with the same letter. Results shown here represent the mean of two independent experiments. Error bars indicate SE. 2019-01-10 08:46:54 Noccaea hyperaccumulator pseudomonas elemental defence zinc cell death glucosinolates