10.6084/m9.figshare.7375568.v1
Jin-Li Zhang
Jin-Li
Zhang
Xiao-Bo Yuan
Xiao-Bo
Yuan
Sun-Jie Chen
Sun-Jie
Chen
Hao-Hao Chen
Hao-Hao
Chen
Nan Xu
Nan
Xu
Wen-Hua Xue
Wen-Hua
Xue
Sheng-Jie Fu
Sheng-Jie
Fu
Chuan-Xi Zhang
Chuan-Xi
Zhang
Hai-Jun Xu
Hai-Jun
Xu
Table S5 from The histone deacetylase NlHDAC1 regulates both female and male fertility in the brown planthopper, <i>Nilaparvata lugens</i>
The Royal Society
2018
histone deacetylase
planthopper
fertility
courtship song
2018-11-22 05:07:02
Dataset
https://rs.figshare.com/articles/dataset/Table_S5_from_The_histone_deacetylase_NlHDAC1_regulates_both_female_and_male_fertility_in_the_brown_planthopper_i_Nilaparvata_lugens_i_/7375568
Histone acetylation is a specific type of chromatin modifications that serves as a key regulatory mechanism for many cellular processes in mammals. However, little is known about its biological function in invertebrates. Here, we identified 12 members of histone deacetylases (NlHDACs) in the brown planthopper (BPH), <i>Nilaparvata lugens</i>. RNAi-mediated silencing assay showed that the <i>NlHdac1</i>, <i>NlHdac3</i> and <i>NlHdac4</i> played critical roles in female fertility via regulating ovary maturation or ovipositor development. Silencing of <i>NlHdac1</i> substantially increased acetylation level of histones H3 and H4 in ovaries, indicating NlHDAC1 is the main histone deacetylase in ovaries of BPH. RNA sequencing (RNA-seq) analysis showed that knockdown of <i>NlHdac1</i> impaired ovary development via multiple signalling pathways including the TOR pathway. Acoustic recording showed that males with <i>NlHdac1</i> knockdown failed to make courtship songs, and thus were unacceptable to wild-type females, resulting in unfertilized eggs. Competition mating assay showed that wild-type females overwhelmingly preferred to mate with control males over <i>NlHdac1</i>-knockdown. These findings improve our understanding of reproductive strategies controlled by HDACs in insects and provide a potential target for pest control.