10.6084/m9.figshare.6235541.v1 Daisuke Noshiro Daisuke Noshiro Toshio Ando Toshio Ando Movie S3 from Substrate protein dependence of GroEL–GroES interaction cycle revealed by high-speed atomic force microscopy imaging The Royal Society 2018 chaperonin GroEL allostery imaging high-speed atomic force microscopy 2018-05-09 05:53:52 Media https://rs.figshare.com/articles/media/Movie_S3_from_Substrate_protein_dependence_of_GroEL_GroES_interaction_cycle_revealed_by_high-speed_atomic_force_microscopy_imaging/6235541 HS-AFM movie of GroEL−GroES interaction during steady-state ATPase reaction cycle in the absence of substrate protein. D490C GroEL biotinylated at Cys490 was immobilized on a surface of tamavidin 2-LPI 2D crystals in a side-on orientation, while GroES was free in the bulk solution. The images were acquired at 4.35 fps for an area of 64 × 80 nm2. Z-scale, 14.5 nm.