10.6084/m9.figshare.6071633.v1
Daisuke Noshiro
Daisuke
Noshiro
Toshio Ando
Toshio
Ando
Movie S1 from Substrate protein dependence of GroEL–GroES interaction cycle revealed by high-speed atomic force microscopy imaging
The Royal Society
2018
chaperonin
GroEL
allostery
imaging
high-speed atomic force microscopy
2018-03-31 11:15:01
Media
https://rs.figshare.com/articles/media/Movie_S1_from_Substrate_protein_dependence_of_GroEL_GroES_interaction_cycle_revealed_by_high-speed_atomic_force_microscopy_imaging/6071633
HS-AFM movie of GroEL−GroES interaction during steady-state ATPase reaction cycle in the presence of DM-MBP. D490C GroEL biotinylated at Cys490 was immobilized on a surface of tamavidin 2-LPI 2D crystals in a side-on orientation, while GroES and denature DM-MBP were free in the bulk solution. The images were acquired at 4.35 fps for an area of 64 × 80 nm2. Z-scale, 14.5 nm.