%0 Online Multimedia %A Noshiro, Daisuke %A Ando, Toshio %D 2018 %T Movie S3 from Substrate protein dependence of GroEL–GroES interaction cycle revealed by high-speed atomic force microscopy imaging %U https://rs.figshare.com/articles/media/Movie_S3_from_Substrate_protein_dependence_of_GroEL_GroES_interaction_cycle_revealed_by_high-speed_atomic_force_microscopy_imaging/6071630 %R 10.6084/m9.figshare.6071630.v1 %2 https://rs.figshare.com/ndownloader/files/10937915 %K chaperonin %K GroEL %K allostery %K imaging %K high-speed atomic force microscopy %X HS-AFM movie of GroEL−GroES interaction during steady-state ATPase reaction cycle in the absence of substrate protein. D490C GroEL biotinylated at Cys490 was immobilized on a surface of tamavidin 2-LPI 2D crystals in a side-on orientation, while GroES was free in the bulk solution. The images were acquired at 4.35 fps for an area of 64 × 80 nm2. Z-scale, 14.5 nm. %I The Royal Society