10.6084/m9.figshare.5999783.v1 Melissa Cui Bender Melissa Cui Bender Caroline Hu Caroline Hu Chris Pelletier Chris Pelletier Robert J. Denver Robert J. Denver Supplemental Fig. 1 from To eat or not to eat: ontogeny of hypothalamic feeding controls and a role for leptin in modulating life-history transition in amphibian tadpoles The Royal Society 2018 metamorphosis tadpole feeding behaviour leptin 2018-03-19 14:28:33 Figure https://rs.figshare.com/articles/figure/Supplemental_Fig_1_from_To_eat_or_not_to_eat_ontogeny_of_hypothalamic_feeding_controls_and_a_role_for_leptin_in_modulating_life-history_transition_in_amphibian_tadpoles/5999783 Dorsal view of <i>Xenopus</i> brain showing the three regions dissected for gene expression analysis by RTqPCR (anatomical drawing from Tuinhof et al.) [1]. <b>B.</b> Western blot of rxLeptin using rabbit anti-rxLeptin serum. We resolved 100 ng of rxLeptin by 10% PAGE before transfer to nitrocellulose for immunoblotting. The rxLeptin, which has poly-histidine and V5 tags, has an expected molecular weight of 21.6 kD.