Bender, Melissa Cui Hu, Caroline Pelletier, Chris Denver, Robert J. Supplemental Fig. 5 from To eat or not to eat: ontogeny of hypothalamic feeding controls and a role for leptin in modulating life-history transition in amphibian tadpoles Expression of the stress and feeding control-related gene corticotropin-releasing factor b (<i>crfb</i>) in three brain regions of <i>X. laevis</i> tadpoles during metamorphosis. We measured changes in <i>crfb</i> mRNA throughout metamorphosis by RTqPCR (see Supplemental Methods). We saw statistically significant changes in <i>crfb</i> mRNA during metamorphosis in all brain regions analyzed. The level of <i>crfb</i> mRNA was highest in the POA/hypothalamus and in the hindbrain/spinal cord, and it increased in these regions throughout metamorphosis beginning at NF stage 58. The expression pattern of <i>crfb</i> mRNA is consistent with the distribution of CRF immunoreactivity in frog brain [2]. The level of <i>crfb</i> mRNA showed a small increase at NF stage 66 in the region of the olfactory bulbs/forebrain (olfactory bulbs/forebrain: F<sub>(4,20)</sub>=5.570, p=0.004; preoptic area/hypothalamus: F<sub>(4,20)</sub>=27.125, p<0.0001; hindbrain/spinal cord: F<sub>(4,20)</sub>=17.323, p<0.0001; ANOVA). Shown are the meansĀ±SEM (n=5/developmental stage). metamorphosis;tadpole;feeding behaviour;leptin 2018-03-19
    https://rs.figshare.com/articles/figure/Supplemental_Fig_5_from_To_eat_or_not_to_eat_ontogeny_of_hypothalamic_feeding_controls_and_a_role_for_leptin_in_modulating_life-history_transition_in_amphibian_tadpoles/5999768
10.6084/m9.figshare.5999768.v1